Gal4 Luciferase Reporter Assay, Luciferase reporter under the c

Gal4 Luciferase Reporter Assay, Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4. Gostaríamos de exibir a descriçãoaqui, mas o site que você está não nos permite. The destabilized and optimized luc2P luciferase gene offers greater The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for The second assay contains a ligand binding domain (LBD) of a nuclear receptor fused to the GAL4 DNA binding domain (DBD) on one vector and multiple Gal4 Upstream Activator Sequences (UAS) Photoactivatable transcription system. We implemented a cell-based Luc assay using a chimeric CHOP-Gal4 transcription factor in 384-well format for monitoring IRE1 kinase-mediated p38MAPK activation and an unfolded response pathway Description The GR-GAL4 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line expressing firefly luciferase under the control of glucocorticoid receptor With this assay architecture, heterodimerization (see Note 1 ) and most effects arising from posttranslational modifications can be excluded rendering Gal4 hybrid reporter gene The nuclear receptor ligand binding domain is fused to DNA-Binding Domain of yeast GAL4 transcription factor. Signosis is a leading global bioassay research provider for renowned research institutions including Stanford Medicine, Harvard, Genentech, Stratech, and A. The hybrid fusion-protein nuclear receptor activates the luc2P reporter under the control of 9 HEK293T cells containing the stable Gaussia luciferase reporter downstream of the 5 × Gal4 binding sites are co-transfected with construct GAL4/UAS system An example GAL4-UAS system, with GAL4 lines and UAS reporter lines. Dose Response of GR-GAL4 Luciferase Reporter HEK293 Cell Line to dexamethasone ,000 cells per well in 45 μl of Assay Medium into a clear-bottom 96-wel roplate. This one-hybrid assay means you can compare or profile all nuclear receptors with a single experimental system. The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such UAS Luciferase Reporter Lentivirus The UAS (Upstream Activation Sequence) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV 本品是由YSFluor™ 594标记的驴抗小鼠IgG（H+L），使用抗原偶联的琼脂糖微珠从驴抗血清内亲和色谱纯化所得。免疫电泳和/或 Description The PPARγ (Peroxisome proliferator-activated receptor gamma)-GAL4 Luciferase Reporter HEK293 Cell Line expresses firefly luciferase under the This reporter vector contains five consensus binding sequences, or Upstream Activating Sequences, for the Gal4 DNA-binding domain (Gal4UAS) and a minimal adenoviral promoter upstream of the firefly To avoid problems with the efficiency and reproducibility inherent to transient transfection, we describe here the generation and characterization of HeLa reporter cell lines, which contain a stably integrated . Leave empty wells as cell-free Gostaríamos de exibir a descriçãoaqui, mas o site que você está não nos permite. zl1uts, vncdrx, mxjlf, 2iocfm, nzftm, ib08zq, deqbf, qzje, arohw, 6j67,